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pdna harbouring gene encoding egfp  (GenScript corporation)
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GenScript corporation pdna harbouring gene encoding egfp
AMP effects on transcription and translation processes. Cell-free <t>eGFP</t> synthesis followed in the absence and presence of antimicrobial peptides (a-f). The cell-free synthesis of eGFP was initiated by supplementing CFPS solution with 6 ng/μL <t>pDNA</t> (a-c) or 80 ng/μL mRNA (d-f) encoding eGFP protein. Continuous lines are the fits (see Materials and Methods). Comparison of EC50 values of AMPs in pDNA- or mRNA-initiated CFPS reaction (g). Error bars are the standard deviations.
Pdna Harbouring Gene Encoding Egfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AMP effects on transcription and translation processes. Cell-free eGFP synthesis followed in the absence and presence of antimicrobial peptides (a-f). The cell-free synthesis of eGFP was initiated by supplementing CFPS solution with 6 ng/μL pDNA (a-c) or 80 ng/μL mRNA (d-f) encoding eGFP protein. Continuous lines are the fits (see Materials and Methods). Comparison of EC50 values of AMPs in pDNA- or mRNA-initiated CFPS reaction (g). Error bars are the standard deviations.

Journal: bioRxiv

Article Title: Modulating Nucleic Acid Phase Transitions as a Mechanism of Action for Cell-Penetrating Antimicrobial Peptides

doi: 10.1101/2023.03.11.532143

Figure Lengend Snippet: AMP effects on transcription and translation processes. Cell-free eGFP synthesis followed in the absence and presence of antimicrobial peptides (a-f). The cell-free synthesis of eGFP was initiated by supplementing CFPS solution with 6 ng/μL pDNA (a-c) or 80 ng/μL mRNA (d-f) encoding eGFP protein. Continuous lines are the fits (see Materials and Methods). Comparison of EC50 values of AMPs in pDNA- or mRNA-initiated CFPS reaction (g). Error bars are the standard deviations.

Article Snippet: Every precaution was taken to avoid contamination with RNases, and RNA samples were kept as 10 μL aliquots at −80 °C to minimise degradation. pDNA harbouring gene encoding eGFP was purchased from GenScript.

Techniques: Comparison